ABSTRACT
<p><b>OBJECTIVE</b>To investigate the safety and therapeutic effect of low dose (1000 U/m(2)) L-asparaginase (L-Asp) in the treatment of children with acute lymphoblastic leukemia (ALL).</p><p><b>METHODS</b>Six patients were treated with low dose L-Asp after previously suffered severe side effects from standard dose L-Asp (5000 - 10,000 U/m(2)). Twenty-eight blood samples were obtained randomly from 5 of them. Plasma asparagine concentration was detected by reverse phase-high performance liquid chromatography (RP-HPLC).</p><p><b>RESULTS</b>All the patients treated with low dose L-Asp showed no any toxic symptoms. The plasma asparagine levels in the patients were all above 5 micromol/L except case 4 (4.91 micromol/L) before receiving L-Asp, and were all decreased below 0.5 micromol/L five days after receiving low dose L-Asp, except case 3 (3.70 micromol/L), the results being like that of receiving standard dose L-Asp.</p><p><b>CONCLUSION</b>Low dose L-Asp has definite efficacy for childhood ALL, while avoids serious side effects from standard dose L-Asp.</p>
Subject(s)
Adolescent , Child , Child, Preschool , Female , Humans , Male , Antineoplastic Agents , Blood , Asparaginase , Blood , Precursor Cell Lymphoblastic Leukemia-Lymphoma , Drug Therapy , Treatment OutcomeABSTRACT
Objective To investigate the protective effects of quercetin on primary cultured SD neonate rats cardiomyocyte injury induced by daunorubicin.Methods Cultured cardiomyocytes were divided into blank groups,DNR groups,DNR+QUE groups,QUE groups.After preincubation of cardiomyocytes for 24 hours,cytoprotection effect was assessed by cell morphous,and cell apoptotic rates determined by flow cytometry,extracellular lactate dehydrogenase(LDH),superoxide dismutase(SOD) and malondialdehyde(MDA).Results Compared with DNR groups,the shedded cardiomyocytes and the cell debris of DNR+QUE groups decreased;the cardiomyocyte apoptosis rates decreased;the content of LDH and MDA decreased(P
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the changes in the activity of Escherichia coli asparaginase (L-asp) and the concentration of asparagines (ASN) in the plasma of the acute lymphoblastic leukemia (ALL) children receiving L-asp containing chemotherapeutic protocol to explore more reasonable usage of L-asp in the treatment of childhood ALL.</p><p><b>METHODS</b>L-asp containing hemotherapy regimen of VDLP was used, in which L-asp (10,000 U/m(2)) was administered intravenously every other day for 10 doses in 15 children with ALL. A total of 340 peripheral blood samples were collected at scheduled time points during the therapy and plasma L-asp activity (by spectrophotometric assay) and asparagines concentration (by RP-HPLC) were measured.</p><p><b>RESULTS</b>During the administration of L-asp, the plasma L-asp activity was increasing gradually peaked after eight doses and then decreased gradually, while the plasma concentration of asparagines maintained in complete or nearly complete depletion status. After the therapy courses finished, a plasma L-asp activity above 100 U/L with asparagines almost complete depletion status was lasting for about seven days.</p><p><b>CONCLUSION</b>The current L-asp containing chemotherapeutic protocols in which L-asp was administered in a dose of 10 000/m(2) intravenously every other day, are efficient enough for the depletion of plasma ASN.</p>
Subject(s)
Adolescent , Child , Child, Preschool , Female , Humans , Male , Antineoplastic Combined Chemotherapy Protocols , Blood , Pharmacokinetics , Therapeutic Uses , Asparaginase , Blood , Pharmacokinetics , Asparagine , Blood , Drug Administration Schedule , Infusions, Intravenous , Precursor Cell Lymphoblastic Leukemia-Lymphoma , Blood , Drug Therapy , Treatment OutcomeABSTRACT
Objective To evaluate the effect of the intermittent deferomamine(DF) therapy on relieving iron overload caused by transfusion in children with ? thalassemia.Methods Sixteen children who were finally diagnosed as ? thalassemia major were treated with deferomamine for 124 times totally to low the iron overload. The serum iron(SI), serum ferritin(SF) and urine ferritin were detected each time with radio-immunity technique and difference was compared before and after treatment. Meanwhile, weather DF involved children′s liver and renal function was observed in whole procedure.Results Iron overload exists in 16 cases of ? thalassemia major children by a long- term hypertransfusion therapy, with average level SI 33.69?6.72 mmol/L,SF 441.19? 54.70 ?g/L,urine ferritin 8.64?6.79 ?g/L. The difference was significant (paired-samples t test,t =6.173 P 0.05).Conclusion The study suggest that intermittent low-dose DF therapy is effective for iron overload caused by transfusion in ? thalassemia children, without apparent side effects.